RSeQC

Read Distribution

Figure 1 : barplot of the read distribution for each samples.

source : nf-core RNAseq MultiQC

Caption:

CDS_Exons refers to the coding part of each exon (excluding the 5’ UTR or 3’ UTR). 5’UTR_Exons refers to the 5’ UTR part of the exons, and 3’UTR_Exons refers to the 3’ UTR part of the exons. Introns are self-explanatory. TSS_up_1kb/1kb-5kb/5kb-10kb are transcription start sites, which are split into three parts: up to 1 kb, 1-5 kb, and 5-10 kb. TES_down_1kb/1kb-5kb/5kb-10kb are transcription end sites, which are split into three parts: down to 1 kb, 1-5 kb, and 5-10 kb. Other_intergenic contains the centromere and other undefined regions.

For RNAseq data, you should see a majority of CDS and UTR ends. However, it is normal to find introns coming from highly expressed genes.